The biology laboratory, classified P2, provides support to users for the preparation of their experiments and to researchers of SOLEIL in the field of health, biology
The biology laboratory is a facility to
- users for the preparation and characterization of biological samples previous to their experiments at the beamlines
- the researches at SOLEIL in the molecular and cellular biology and biochemistry fields (Section scientifique : Biologie, santé, Heliobio)
Location of the biology laboratory
Team
Functioning
- Common to 5 beamlines : DISCO, PROXIMA-1, Proxima-2A, SMIS and SWING
- Security access by badge
- Open 24h/24h to external users/scientists who apply for
- The laboratory group will be present only at working hours. However, red classified experiments need the present of a biology laboratory or beamline scientist. In that case their disponibilities has to be verify before user’s arrival
Common laboratory equipment and consumables
- Cold room
- Hood
- Safety cabinet (acids/bases and solvents)
- Benches
- Sinks
- Autoclave
- Lab washer
- Ice machine
- Microwave
- Freezer -20° et -80°
- Balance (precision 10mg)
- Balance (precision 1/100mg)
- Vacuum pump
- Micropipettes
- Ultra pure water
- pH meter
- Shakers, mini spin
- Water bath
- Burner
- Hot plate stirrer
- Vortex
- Glassware (beaker,…)
- Small materials (tubes,…)
- Safety equipment (gloves,…)
- Reagents/Solvents
- Gas
Molecular Biology and Biochemistry Equipment
- Thermal cycler
- Electrophoresis system
- Blotting system
- Speed vac
- Bacteriological oven
- High performance centrifuge
- Micro-centrifuges
- Sonicator
- Orbital shaking incubator
- FPLC
- Imaging system
- Spectrophotometer UV/Vis
Services
The laboratory staff will provide scientific (advice/experience) and technical support (faisabilité/setting up of experiments). They will make available to the users the classical equipments and materials of a biology laboratory.
Access application
- At the proposal submission in SUN set, a specify requirement of the biology laboratory has to be declared by the main proposer.
If the project is accepted, the main proposer must contact the laboratory manager.
- At least one month before the arrival the main proposer confirms the access request, on-line on SUN set and details the needs:
- planning activities to be carried out in the laboratory
- scientific equipment to be used among available ones
- biological and biochemical materials to be used
- request for technical supportValidation of the application will be done by the laboratory manager.
Safety requirements
- All samples and biological and biochemical materials necessary for sample preparation have to be declared on-line (cf. "samples and substances declaration" section).
- Only biological and biochemical materials approved by the safety group of SOLEIL synchrotron can be used.
- Each sample must be identified and must have been specifically declared according to its nature (cf. Samples and substances declaration).
- Each sample or handled biological and biochemical product has to be labelled. The following information has to be mentioned:
- the name of the product
- the date
- the name of the project and phone number of the person to be contacted.
- Warnings and indications in the laboratory must be respected.
- Individual protection against biohazardous substances must be used for any handling (lab-coats, gloves, glasses, closed shoes and working under fume hood if necessary).
- Handling biohazardous chemicals alone in the laboratory is forbidden.
- Biological and biochemical wastes must be disposed of in suitable containers (separation of flammable products, acids, basics, solvents, carcinogenic, harmful or toxics substances…)
- Any drinking or any eating in the room is forbidden
Research projects:
Replicative complex in Paramyxovirus family: Pneumovirus, Metapneumovirus and Respirovirus.
Collaborations:
Félix Rey, Structural Virology
Institut Pasteur (Paris)
Jean-François Eléouët, Molecular Virology and Immunology
INRA (Jouy-en-Josas)
Recent Publications:
Tran TL, Castagne N, Bhella D, Varela PF, Bernard J, Chilmonczyk S, Berkenkamp S, Benhamo V, Grznarova K, Grosclaude J, Nespoulos C, Rey FA, Eleouet JF.
J Gen Virol. 2007 Jan; 88(Pt 1):196-206
