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Biology laboratory

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The biology laboratory, classified P2, provides support to users for the preparation of their experiments and to researchers of SOLEIL in the field of health, biology

The biology laboratory is a facility to

  • users for the preparation and characterization of biological samples previous to their experiments at the beamlines
  • the researches at SOLEIL in the molecular and cellular biology and biochemistry fields (Section scientifique : Biologie, santé, Heliobio)

 

 

Location of the biology laboratory

Team

DAVID
DAVID Gabriel
PINEAU
PINEAU Blandine
THRO

Functioning

  • Common to 5 beamlines : DISCO, PROXIMA-1, Proxima-2A, SMIS and SWING
  • Security access by badge
  • Open 24h/24h to external users/scientists who apply for
  • The laboratory group will be present only at working hours. However, red classified experiments need the present of a biology laboratory or beamline scientist. In that case their disponibilities has to be verify before user’s arrival

Common laboratory equipment and consumables

  • Cold room
  • Hood
  • Safety cabinet (acids/bases and solvents)
  • Benches
  • Sinks
  • Autoclave
  • Lab washer
  • Ice machine
  • Microwave
  • Freezer -20° et -80°
  • Balance (precision 10mg)
  • Balance (precision 1/100mg)
  • Vacuum pump
  • Micropipettes
  • Ultra pure water
  • pH meter
  • Shakers, mini spin
  • Water bath
  • Burner
  • Hot plate stirrer
  • Vortex
  • Glassware (beaker,…)
  • Small materials (tubes,…)
  • Safety equipment (gloves,…)
  • Reagents/Solvents
  • Gas

Molecular Biology and Biochemistry Equipment

  • Thermal cycler
  • Electrophoresis system
  • Blotting system
  • Speed vac
  • Bacteriological oven
  • High performance centrifuge
  • Micro-centrifuges
  • Sonicator
  • Orbital shaking incubator
  • FPLC
  • Imaging system
  • Spectrophotometer UV/Vis

Services

The laboratory staff will provide scientific (advice/experience) and technical support (faisabilité/setting up of experiments). They will make available to the users the classical equipments and materials of a biology laboratory.

Access application

  • At the proposal submission in SUN set, a specify requirement of the biology laboratory has to be declared by the main proposer.

    If the project is accepted, the main proposer must contact the laboratory manager.

  • At least one month before the arrival the main proposer confirms the access request, on-line on SUN set and details the needs: 

    - planning activities to be carried out in the laboratory 
    - scientific equipment to be used among available ones 
    - biological and biochemical materials to be used
    - request for technical support

    Validation of the application will be done by the laboratory manager.

Safety requirements

  • All samples and biological and biochemical materials necessary for sample preparation have to be declared on-line (cf. "samples and substances declaration" section). 
     
  • Only biological and biochemical materials approved by the safety group of SOLEIL synchrotron can be used.
     
  • Each sample must be identified and must have been specifically declared according to its nature (cf. Samples and substances declaration).
     
  • Each sample or handled biological and biochemical product has to be labelled. The following information has to be mentioned: 

- the name of the product
- the date
- the name of the project and phone number of the person to be contacted.  

  • Warnings and indications in the laboratory must be respected.
     
  • Individual protection against biohazardous substances must be used for any handling (lab-coats, gloves, glasses, closed shoes and working under fume hood if necessary).
     
  • Handling biohazardous chemicals alone in the laboratory is forbidden.
     
  • Biological and biochemical wastes must be disposed of in suitable containers (separation of flammable products, acids, basics, solvents, carcinogenic, harmful or toxics substances…)
     
  • Any drinking or any eating in the room is forbidden

 Research projects:

Replicative complex in Paramyxovirus family: Pneumovirus, Metapneumovirus and Respirovirus. 
 

 Collaborations:

Félix Rey, Structural Virology
Institut Pasteur (Paris)

Jean-François Eléouët, Molecular Virology and Immunology 
INRA (Jouy-en-Josas) 
 

 Recent Publications: 

Tran TL, Castagne N, Bhella D, Varela PF, Bernard J, Chilmonczyk S, Berkenkamp S, Benhamo V, Grznarova K, Grosclaude J, Nespoulos C, Rey FA, Eleouet JF. 
J Gen Virol. 2007 Jan; 88(Pt 1):196-206