Aller au menu principal Aller au contenu principal

PROXIMA-2A

Partager
PROXIMA2A logo

Welcome to PROXIMA-2A!

A leading light for micro-crystallography.

PX2-A wide-angle panorama phot

PROXIMA 2-A (PX2-A) is a microfocus beamline for biological crystallography at Synchrotron SOLEIL. The beamline is highly automated and designed to help you tackle the most challenging structural targets and biological systems. 

PX2-A is rapidly tunable over the energy range 6-17 keV making the most commonly used absorption edges accessible for anomalous diffraction experiments. 

The beamline is equipped with a high capacity sample-changing system, high performance micro-diffractometer, KETEK X-ray fluorescence detector, and an EIGER X 9M photon-counting area detector.

See also Biology Health : HelioBio

L'équipe

CICCONE
CICCONE Lidia
FOX
FOX Gavin
Scientifique de Ligne De Lumière
JEANGERARD
JEANGERARD Damien
SAVKO
SAVKO Martin
Scientifique de Ligne De Lumière
SHEPARD
SHEPARD William
Responsable Ligne De Lumière
SIMPKIN
SIMPKIN Adam *
Doctorant
Duran Denis
Assistant Engineer
MEIJERS Rob
Beamline Scientist

Offres d'emploi

Please contact William SHEPARD for more information.

Données techniques

Energy range

Between 5 KeV and 15 KeV

Energy Resolution

~0.0002 (Si 111)

Source

Two U24 canted undulators (4.5 mrad)

Flux @ first optical element

White beam – depends on undulator settings

Optics

cryogenically cooled channel cut crystal monochromator, a convex prefocussing mirror and a Kirkpatrick-Baez pair of focussing mirrors

Polarization

Linear

Thématiques scientifiques

Micro-crystallography

Small and weakly diffracting crystals

SAD and MAD phasing

Phasing with a range of heavy atoms, including sulphur

Plate screening

Screening of crystallization trays

En recherche interne à SOLEIL, la ligne de lumière PROXIMA-2A est rattachée à la section scientifique :

Section scientifique SOLEIL Biologie, santé, Héliobio

 

Before visiting PX2-A

Please contact your LC before departing your lab.

  • How many samples?
  • Which X-ray wavelengths or heavy atom derivatives?
     

Sending dewars

Dewars should be sent to your Local Contact (one of the people below):

  • W. Shepard
  •  G. Fox
  • M. Savko

SOLEIL's address is:

PROXIMA 2A
Synchrotron SOLEIL
L'Orme des Merisiers
Saint-Aubin, BP 48
91192 GIF-sur-YVETTE CEDEX
FRANCE

Please notify your Local Contact, so that (s)he can refill your dewar upon arrival!

The return of shipping dewars must be pre-paid. BIOSTRUCT-X participants will be reimbursed when they submit their shipping dewar receipts to the Missions Bureau at SOLEIL.

Types of samples (and videos)

PROXIMA-2A will take ANY & ALL samples! This includes the BIG, the SMALL and the UGLY!

  • big > 10 microns
  • small < 10 microns
  • ugly = split, fractured and clumped crystals, because these are sometimes made up of nice micro-crystals!

 
Types of sample pins :

- SPINE standard pins only

  • pin length = 22 mm (from loop to base)
  • goniometer horizontal range = -25 to +5 mm
     

- Manual & robot mounting

  • Suitable pins are Hampton, Mitegen & Molecular Dimensions
  • BEWARE: the goniometer axis is horzontal and it can not be inclined for easy cryo-transfers

PLEASE PRACTICE BEFORE MOUNTING YOUR FAVORITE CRYSTAL!

- Robotic mounting
A short video explaining mounting procedures :

A further video showing the use of a dry shipper and sample recovery :

During your visit

- Start & finish times for PROXIMA-2A users:

  • 9:00 am – 8:00 am
     

- Equipment & accessories available to users include:

  • Microscopes, SPINE pins, loops & wands for fishing crystals
  • Cryo-equipment - liquid nitrogen, handling dewars, cryo-tongs, gloves, etc...
  • ALL OF THIS EQUIPMENT MUST REMAIN ON THE BEAMLINE!
     

- Data Collection Control

Users launch their experiments via the MXCuBE GUI

  • beam location
  • sample transfers (manual & robotic)
  • crystal centring
  • data collections
  • energy changes, Escans & XFE spectra
     

- Accessible X-ray wavelengths

  • 7 – 15 keV
     

- Accessible absorption edges

  • K-edges Fe – Kr
  • L3-edges Gd - Rn
     

- Typical data collection times

  • Less than 3s per image (ADSC Q315r)
  • 5-20 minutes per standard data collection depending upon strategy
  • most crystals require 0.5s per 0.5 degree oscillation angle
     

- Visualising diffraction images

  • ADXV
     

- Data processing

  • XDSME
     

- Canteen Opening Hours

  • Closed during shutdowns
  • Weekdays (Monday - Friday) : Breakfast 7:30 - 9:00   Lunch 11:45 - 13:45      Dinner 19:00 - 20:30
  • Weekends& Holidays : Breakfast 7:30 - 9:00     Lunch 11:45 - 13:45         Dinner 18:00 - 19:30

After your experiment

- Recuperating your data

  • download on to external disk drives via USB2 port
  • if possible, use the automated script "synchrosync" (Cntrl-C to stop, and then dimount the drive).

- Remove ALL of your samples

- Sign the Safety Approval Sheet

Important: Please send to us any slides, images or publications of your results...
the more the merrier! Bon courage!

Documents à télécharger