Welcome to PROXIMA-2A!
A leading light for micro-crystallography.
PROXIMA 2-A (PX2-A) is a microfocus beamline for biological crystallography at Synchrotron SOLEIL. The beamline is highly automated and designed to help you tackle the most challenging structural targets and biological systems.
PX2-A is rapidly tunable over the energy range 6-17 keV making the most commonly used absorption edges accessible for anomalous diffraction experiments.
The beamline is equipped with a high capacity sample-changing system, high performance micro-diffractometer, KETEK X-ray fluorescence detector, and an EIGER X 9M photon-counting area detector.
See also Biology Health : HelioBio
L'équipe
Nos derniers tweets
- ELI Beams & IBT-Biocev scientists successfully test the EIGER 1M detector at 3kHz on @PROXIMA2A: Low flux MX is pos… https://t.co/NRYLHDaMrN
- Raphael de Wijn @unistra & Jennifer Roche @AFMBLab shooting microcrystals @Proxima2A in ChipX-3 @microfluidics https://t.co/SHcyzVeJJX
- RT @synchroSOLEIL: Des colles moléculaires pour assembler les protéines : vers une administration ciblée des médicaments ?… https://t.co/KS1PlZSAlc
- RT @synchroSOLEIL: “Myosin 7 and its adaptors link cadherins to actin”: Three SOLEIL_beamlines involved @PROXIMA_1 @Proxima2A #SWING… https://t.co/zxrfknstvn
Offres d'emploi
Please contact William SHEPARD for more information.
Collaborations
Données techniques
Between 5 KeV and 15 KeV
~0.0002 (Si 111)
Two U24 canted undulators (4.5 mrad)
White beam – depends on undulator settings
cryogenically cooled channel cut crystal monochromator, a convex prefocussing mirror and a Kirkpatrick-Baez pair of focussing mirrors
Linear
Thématiques scientifiques
| Micro-crystallography |
Small and weakly diffracting crystals |
|---|---|
| SAD and MAD phasing |
Phasing with a range of heavy atoms, including sulphur |
| Plate screening |
Screening of crystallization trays |
En recherche interne à SOLEIL, la ligne de lumière PROXIMA-2A est rattachée à la section scientifique :
| Section scientifique SOLEIL | Biologie, santé, Héliobio |
|---|
Before visiting PX2-A
Please contact your LC before departing your lab.
- How many samples?
- Which X-ray wavelengths or heavy atom derivatives?
Sending dewars
Dewars should be sent to your Local Contact (one of the people below):
- W. Shepard
- G. Fox
- M. Savko
SOLEIL's address is:
PROXIMA 2A
Synchrotron SOLEIL
L'Orme des Merisiers
Saint-Aubin, BP 48
91192 GIF-sur-YVETTE CEDEX
FRANCE
Please notify your Local Contact, so that (s)he can refill your dewar upon arrival!
The return of shipping dewars must be pre-paid. BIOSTRUCT-X participants will be reimbursed when they submit their shipping dewar receipts to the Missions Bureau at SOLEIL.
Types of samples (and videos)
PROXIMA-2A will take ANY & ALL samples! This includes the BIG, the SMALL and the UGLY!
- big > 10 microns
- small < 10 microns
- ugly = split, fractured and clumped crystals, because these are sometimes made up of nice micro-crystals!
Types of sample pins :
- SPINE standard pins only
- pin length = 22 mm (from loop to base)
- goniometer horizontal range = -25 to +5 mm
- Manual & robot mounting
- Suitable pins are Hampton, Mitegen & Molecular Dimensions
- BEWARE: the goniometer axis is horzontal and it can not be inclined for easy cryo-transfers
PLEASE PRACTICE BEFORE MOUNTING YOUR FAVORITE CRYSTAL!
- Robotic mounting
A short video explaining mounting procedures :
A further video showing the use of a dry shipper and sample recovery :
During your visit
- Start & finish times for PROXIMA-2A users:
- 9:00 am – 8:00 am
- Equipment & accessories available to users include:
- Microscopes, SPINE pins, loops & wands for fishing crystals
- Cryo-equipment - liquid nitrogen, handling dewars, cryo-tongs, gloves, etc...
- ALL OF THIS EQUIPMENT MUST REMAIN ON THE BEAMLINE!
- Data Collection Control
Users launch their experiments via the MXCuBE GUI
- beam location
- sample transfers (manual & robotic)
- crystal centring
- data collections
- energy changes, Escans & XFE spectra
- Accessible X-ray wavelengths
- 7 – 15 keV
- Accessible absorption edges
- K-edges Fe – Kr
- L3-edges Gd - Rn
- Typical data collection times
- Less than 3s per image (ADSC Q315r)
- 5-20 minutes per standard data collection depending upon strategy
- most crystals require 0.5s per 0.5 degree oscillation angle
- Visualising diffraction images
- ADXV
- Data processing
- XDSME
- Canteen Opening Hours
- Closed during shutdowns
- Weekdays (Monday - Friday) : Breakfast 7:30 - 9:00 Lunch 11:45 - 13:45 Dinner 19:00 - 20:30
- Weekends& Holidays : Breakfast 7:30 - 9:00 Lunch 11:45 - 13:45 Dinner 18:00 - 19:30
After your experiment
- Recuperating your data
- download on to external disk drives via USB2 port
- if possible, use the automated script "synchrosync" (Cntrl-C to stop, and then dimount the drive).
- Remove ALL of your samples
- Sign the Safety Approval Sheet
Important: Please send to us any slides, images or publications of your results...
the more the merrier! Bon courage!
