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SWING, Small and Wide angle X-ray scattering
Pour l'étude d'échantillons à structure complexe : macromolécules, nanomatériaux, tissus...

 

Pour plus d'informations, merci de vister le site en anglais

For more informations, please visit the english website

 

Fournissant des informations sur la structure de la matière à des échelles comprises entre le nanomètre et le micromètre, la ligne SWING permettra de répondre aux nombreuses questions liées à la matière molle, à la conformation de macromolécules en solution et aux matières composites en sciences des matériaux.

Cette installation expérimentale permettra d'effectuer simultanément des mesures de diffusion de rayons X aux petits angles (SAXS) et grands angles (WAXS) dans la gamme d'énergie de 5-16 keV aussi bien que des mesures de diffusion en incidence rasante (GISAXS). Des expériences de diffusion anomale seront également possibles. L'accent sera mis sur la variété d'échantillons qui peuvent être étudiés, solutions, gels, solides amorphes, solides cristallisés et sur la diversité des environnements correspondants.

L'équipe

BARANOWSKI
BARANOWSKI Maciej
Post-Doctorant
BIZIEN
BIZIEN Thomas
Scientifique de Ligne De Lumière
CASSAN
CASSAN Valentin
GIBERT
GIBERT Arnaud
PEREZ
PEREZ Javier
Responsable Ligne De Lumière
THUREAU
THUREAU Aurelien
Scientifique de Ligne De Lumière
Meneau Florian
Brazilian Synchrotron Light Laboratory (LNLS), Campinas
Roblin Pierre
Laboratory of Chemical Engineering , Toulouse
Galindo-Gonzales Cecilia
Koutsioupas Alexandros
Evrard Guillaume

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Données techniques

Gamme d'énergie

Between ~5 and 16 keV

Résolution en énergie

~2 eV

Source

In-vacuum U20 undulator.
Source Size (sigma, μm): 388 (H) x 8.1 (V)
Source Divergence (sigma, μrad): 14.5 (H) x 4.6 (V)

Optiques

Diaphragm at 11.7 m (1x0.5 mm2
Fixed exit DCM Si111at 20 m
Fixed incidence focusing KB at 22.5 m 
Sample position : 30 - 32 m
Detector / Sample Distance : 0.5 – 6.5 m

Environnements d'échantillon

see Set-up environments

Taille du faisceau

Between 20x20µm2 (zone plate) to 450x20 μm2 FWHM in the experimental hutch

Flux sur l'échantillon

1.1013ph/s @7keV, 1.1012 ph/s @16keV (with 500 mA ring)

Détecteurs

SAXS:

  • Eiger 4M (Dectris)

WAXS: 

  • Merlin Quantum Detector, Detection area 28x28 mm2
Chambre de détection

Under primary vacuum, SAXS detector positions :
- 0.20 / + 0. 20 m (horiz), -0.20 / +0.20 (vert), 0.5 m / +6 m (along X-rays).

Dedicated sample environments have been developed to provide scientific communities with optimized experimental conditions.  For soft-condensed matter, numerous devices are available (home-made capillaries holders, Linkam THMS600, Anton Paar rheometer with custom-made Couette cells …). For structural biology, a unique SEC-SAXS HPLC system is routinely used for online purification connected to a quartz capillary placed under vacuum cell. Here are presented the most used devices for both soft-condensed matter and structural biology.

HPLC: SEC-SAXS

  • Sample volume: 10 to 100µL
  • Sample Concentration: 1 to 30mg/mL
  • Two independent circuits of purification (can connect two columns with 2 different buffers).
  • Combination of the SEC-SAXS system with MALS and RI is now available at user request.
  • Automated workflows for injections and data analysis
  • IspyB database

 

AutoSampler for BioSaxs

  • Sample volume: 10 to 50µL
  • Sample Concentration: 0.2 to 10mg/mL
  • Sample every 2.5 min (pipetting, injection, measurement, cleaning and drying).
  • 96 vials capacity
  • No dead time between batch and SEC-SAXS experiments
  • Thermalized vials holder (5-10 to 60 °C)
  • Injection rates of : 10 to 100 µl.min-1
  • Injection possible up to 30 % glycerol in water / 250 g.l-1 BSA

 

High Pressure

description will arrive soon

Static Capillaries Holder

  • Horizontal and Vertical positions
  • 20 capillaries of Ø 1,5 mm (Ø 1 mm possible)
  • 5 mm high scanning windows behind the capillaries
  • 5 mm gap between capillaries
  • Thermalization between 5-60 °C
  • Automated workflows for injections and data analysis

Circulation Capillaries Holder

Circulation Capillaries Holder
  • 3 capillaries of Ø 1,5 mm
  • Capillaries are vertically spaced of 15 mm
  • 20 mm horizontal scanning length
  • Thermalization between 5-60 °C

Circulation through the capillaries can be done using various setups :

  • Peristaltic pump
  • Swing injection robot
  • Syringe driver
  • Manual injection

 

Gel Holder

description will arrive soon

Mixing and pipetting cell

Mixing and pipetting cell
  • 5-10 ml mixing cell
  • sample pipetting through a Ø 1,5 mm quartz capillary
  • Possible Injection into the mixing cell through an injecting hole synchronized with detection
  • Thermalization possible between 5-10 °C to 60 °C
  • Automated workflows for injections and data analysis

The sample is drawn in the capillary only and at the required height. After X-ray exposition, it’s reinjected into the mixing cell.

 

Linkam

Linkam
  • Linkam THMS600
  • Temperature range: -196 to 600 °C
  • Up to 150°C/min heating and 100°C/min Cooling
  • Capillaries have to be Ø 1,5 mm maximum and cut in order to reach 20 mm length otherwise it won’t fit in the linkam cell
  • Dedicated linkam software

 

Traction Cell

Traction Cell
  • Max. Working load: 111 N
  • Max. working length : 6 mm
  • Resolution 0.05 N
  • Step : 0,5 µm

 

Rheometer

Rheometer

Rheometer Anton Paar MCR 501

  • X-ray transparent Couette cells
  • Couette cells are of 10 mm radius, a height of 17 mm and gaps values (Re – Ri) of 0.5 or 0.2 mm
  • Sample Volume is of about 1 ml

 

Stop-Flow (+MALS)

description will arrive soon

Micro-fluidic

description will arrive soon

BioSaxs

Before Coming

What you have to bring

                We use Agilent standards vials and inserts for the HPLC device and the Auto Sampler. We lend you the vials and the caps but you have to buy you own inserts (Agilent reference: 5181-1270). In any case, we can give some used inserts that you can wash and dry.

                The minimum quantity of buffer is 40ml for one injection (10mL of system purge + 20 mL of column equilibration + 10mL of elution). We will recommande you to bring much more (100mL to 500mL) to obtain a better equilibration of the column and to induce less stress in case of trouble.

                To make your backups directly on the line, a Windows compatible hard disk is required. However, you can still download all your data a posteriori via the sunset[Experiment Data][Soleil Data Retrivial].

What we can lend you

                The biology laboratory (link) provides support to users for the preparation of their experiments. For any question, please ask the laboratory staff.

                We recommend you to bring your own analytical columns for SEC-SAXS. We have also a wide selection of column for users. All these columns have been calibrated:

BioSec3-100

Column Volume:5mL

Mass Range: 0.1 to 100kDa

pH Range: 2-8.5

biosec3-100 elution profile

AdvBioSec 2.7-300

Column Volume:5mL

Mass Range: 5 to 1250kDa

pH Range: 2-8.5

biosec Advance 2.7-300 elution profile

BioSec3-300

Column Volume:5mL

Mass Range: 5 to 1250kDa

pH Range: 2-8.5

biosec 3-300 elution profile

BioSec 5-1000

Column Volume:5mL

Mass Range: 50 to 7500kDa

pH Range: 2-8.5

biosec 5-1000 elution profile

BioSec 5-2000

Column Volume:5mL

Mass Range: >10000kDa

pH Range: 2-8.5

biosec 5-2000 elution profile

During your session

The session includes beamline set up installation. It’s recommended to discuss with your local contact before coming to the beamline to save time during installation.

Running experiments

Our GUI (passerelle) allows automatic data collections of multiples simple in a row. For direct data monitoring and security check, an others GUI (Cook) is installed.

The local contact will show you how to use these GUI.

Data Processing and analysis: Foxtrot & AutoBioSAXS

All the images produced on the Swing beamline can be process by homemade software (Foxtrot) in collaboration with Xenocs. You can ask for it by email to Xenocs by mail.

You can find here (new link) a tutorial for processing SEC-SAXS experiments.

For SEC-SAXS and BioSaxs AutoSampler, an automatic workflow for data analysis is available. This workflow takes raw images as input files and sends all results curves into IspyB database. Furthermore all intermediates files are available for users and could be reloading in our Foxtrot application.

 Living area

We have a living area where you can use coffee machine, Sofa, fridge for food and beverages, micro wave and table. Wifi is available using your sunset login and password or using Eduroam network.

This area is sample free and please clean it after your session.

After your session

Please don’t forget to fill the end of report in the sunset webpage.

All your datas are available using the Soleil data Retrieval via the sunset web page.

ChemSaxs

During your session

The session includes beamline set up installation. It’s recommended to discuss with your local contact before coming to the beamline to save time during installation.

Running experiments

Our GUI (passerelle) allows an “on demand” complex data collections strategies. For most of the sample environment, a workflow is already written. For direct data monitoring and security check, an others GUI (Cook) is installed. The local contact will show you how to use these GUI.

Data Processing and analysis: Foxtrot & AutoChemSAXS

All the images produced on the Swing beamline can be process by homemade software in collaboration with Xenocs. You can ask for it by email to Xenocs (link).

 Living area

We have a living area where you have coffee machine, Sofa, fridge for food and beverages, micro wave and table. Wifi is available using your sunset login and password or using Eduroam network.

This area is sample free and please cleans it after your session.

After your session

Please don’t forget to fill the end of report in the sunset webpage.

All your datas are available using the Soleil data Retrieval via the sunset web page.

en cours...

en cours...

Listes des logiciels développés par la ligne SWING.

Programme Foxtrot

Programme Foxtrot (Réduction et analyse des données)

Demande par mail

Tutoriel pour Foxtrot :
Foxtrot : F.A.Q.

Programme Denfert

Programme Denfert (Détermination ab initio d'enveloppes moléculaires, incluant une couche d'hydratation)
version 2.0 (June 2015)

Denfert : F.A.Q.
A zip archive containing executables for Mac, Linux and Windows together with a test example may be downloaded by clicking here.

... a program for the ab initio “dummy-atom” structural modeling of Biological Macromolecules including the contribution of their inherent hydration layer.

Developed by Alexandros Koutsioubas and Javier Pérez

If you use DENFERT, please cite :

A. Koutsioubas & J. Pérez, Journal of Applied Crystallography (2013) 46, 1884 and A. Koutsioubas, S. Jaksch & J.Pérez, J. (2016). J. Appl. Cryst. 49  (doi:10.1107/S1600576716003393)

Brief Description

DENFERT is implementing a simulated annealing algorithm similar to DAMMIN program by D. Svergun (Biophys. J. 76, 2879-2886) for the restoration of low-resolution structural info of bio-molecules from SAXS and SANS data. The major advantage of DENFERT is that the hydration layer around bio-molecules is taken into account by introducing a second type of beads (hydration beads) in the model.

In the top figure, we see an example of the shape restoration of Lysozyme from SAXS data using DENFERT. A cartoon representation of the crystallographic structure is also presented for comparison. The bottom figure depicts additionally the hydration layer around the reconstructed protein shape.

PDF icon denfert manual (126.1 Ko)

Dadimodo

Dadimodo web server

DADIMODO is a program for refining atomic models of multidomain proteins or complexes against small-angle X-ray scattering data. Domain structures are mainly kept rigid and can be user defined. Stepwise generic conformational changes are applied cyclically in a stochastic optimization algorithm that performs a search in the protein conformation space. The algorithmic structure guarantees that a physically acceptable full atomic model of the structure is present at all stages of the optimization.

Fichier manual (13.19 Ko)