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PROXIMA-2A

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Welcome to PROXIMA-2A! 
A leading light for biology and micro-crystallography.

PROXIMA 2A (PX2-A) is a microfocus beamline dedicated to biological crystallography and innovative micro-beam methodologies. Opened to the scientific community since 2013, the topics treated on the beamline go beyond standard protein crystallography and include drug discovery, membrane proteins, virus crystallography, small molecule crystallography, powder diffraction and even crystalluria.   The beamline is highly automated and designed to help scientists tackle the most challenging structural targets and biological systems. The X-ray energy is rapidly tunable over a wide range (6-18 keV) making the most commonly used absorption edges accessible for anomalous diffraction experiments.  The end station is equipped with a high capacity sample-changing system (148 SPINE pins), a high performance micro-diffractometer with a mini-kappa (MD2), an X-ray fluorescence detector (KETEK), and a fast, low-noise, photon-counting, area detector - the EIGER X 9M (238 fps in 9M mode, 750 fps in 4M mode).

See also Biology Health : HelioBio

Team

DE-ZITTER
JEANGERARD
JEANGERARD Damien
SAVKO
SAVKO Martin
Scientifique de Ligne De Lumière
SHEPARD
SHEPARD William
Responsable Ligne De Lumière
SIMPKIN
SIMPKIN Adam *
Doctorant
SIRIGU
SIRIGU Serena
Scientifique de Ligne De Lumière
DURAN Denis
Assistant Engineer
MEIJERS Rob
Beamline Scientist
SIMPKIN Adam
Doctoral Student

Jobs opportunities

Please contact William SHEPARD for more information.

Technical data

Energy range

6 - 18 keV

Energy Resolution

~0.0002 (Si 111)

Source

Two U24 canted undulators (4.5 mrad)

Flux @ first optical element

White beam – depends on undulator settings

Optics

Cryogenically cooled channel cut crystal monochromator, a convex prefocussing mirror and a Kirkpatrick-Baez pair of focussing mirrors

Polarization

Linear

Detectors

EIGER X 9M area detector for X-ray diffraction images (238 fps in 9M mode, 750 fps in 4M mode)

Ketek SDD detector for X-ray fluorescence scans

Diamond scCVD and Ti foil XBPMs

High resolution X-ray camera (SOLEIL in-house design)

Scientific opportunities

Micro-crystallography

Small and weakly diffracting crystals

SAD and MAD phasing

Phasing with a range of heavy atoms, including sulphur

Plate screening

Screening of crystallization trays

 

Before visiting PX2-A

Please contact your LC before departing your lab.

Sending Dewars

For REMOTE ACCESS users, please send a hard disk drive (HDD 1-2 To) with your Dewar.

Dewars should be sent to your Local Contact (one of the people below):

  • William SHEPARD (Beamline Responsable)
  • Lidia CICCONE
  • Martin SAVKO

SOLEIL's address is:

PROXIMA 2A
Synchrotron SOLEIL
L'Orme des Merisiers
Saint-Aubin, BP 48
91192 GIF-sur-YVETTE CEDEX
FRANCE

Please notify your Local Contact, so that (s)he can refill your dewar upon arrival!

The return of shipping dewars shall be paid by the user. Please provide a FEDEX or DHL account number to your Local Contact. Click here to download a copy of the Dewar Return Form.

Types of samples (and videos)

PROXIMA-2A will take ANY & ALL samples! This includes the BIG, the SMALL and the UGLY!

  • big > 10 microns
  • small < 10 microns
  • ugly = split, fractured and clumped crystals, because these are sometimes made up of nice micro-crystals!

 
Types of sample pins :

- SPINE standard pins only

  • pin length = 22 mm (from loop to base)
  • goniometer horizontal range = -25 to +5 mm
     

- Manual mounting

  • Suitable pins are Hampton, Mitegen & Molecular Dimensions
  • Goniometer now contains a mini-kappa, which can incline the smart magnet for easy cryo-transfers (set omega = 0° and kappa = 30 - 60°)

- Robot mounting

  • The CATS robotic sample changer can hold up to nine (9) uni-pucks which each hold 16 SPINE pins, for a total of 144 pins
  • Suitable pins are Hampton, Mitegen & Molecular Dimensions

A short video explaining mounting procedures :

A further video showing the use of a dry shipper and sample recovery :

During your visit

- Start & finish times for PROXIMA-2A users:

  • 8:30 am – 8:00 am
     

- Equipment & accessories available to users include:

  • Microscopes, SPINE pins, loops & wands for fishing crystals
  • Cryo-equipment - liquid nitrogen, handling dewars, cryo-tongs, gloves, etc...
  • ALL OF THIS EQUIPMENT MUST REMAIN ON THE BEAMLINE!
     

- Data Collection Control: Users launch their experiments via the MXCuBE GUI. See DOWNLOADS section on the home page for the PX2-A MXCuBE users guide, or click here (PX2-A_MXCUBE_USERS_GUIDE). 

  • beam location
  • sample transfers (manual & robotic)
  • crystal centring
  • data collections
  • energy changes, Escans & XFE spectra
  • Mesh, Grid and X-ray centering scans
     

- Accessible X-ray wavelengths

  • 6 – 18 keV
     

- Accessible absorption edges

  • K-edges Cr – Zr
  • L3-edges Ce - U
     

- Typical data collection times

  • Minimum exposure time 4.2 ms per frame (238 fps), nominal exposure time 25 ms per frame (40 fps)
  • 90s per nominal data collection rate (25 ms per frame, 0.1 degrees per frame, 3600 frames)
  • most crystals require 25 ms per 0.1 degrees oscillation angle
     

- Visualising diffraction images

  • ALBULA
  • ADXV
     

- Data processing

  • XDSME
     

- Canteen Opening Hours

  • Closed during shutdowns
  • Weekdays (Monday - Friday) : Breakfast 7:30 - 9:00   Lunch 11:45 - 13:45      Dinner 19:00 - 20:30
  • Weekends& Holidays : Breakfast 7:30 - 9:00     Lunch 11:45 - 13:45         Dinner 18:00 - 19:30

After your experiment

- Recuperating your data

  • download on to external disk drives via USB2 port
  • if possible, use the automated script "altsync" (Cntrl-C to stop, and then dimount the drive).
  • Only low volumes of data (e.g. MTZ files) can be practically downloaded via the SOLEIL Data Retrieval system

- Remove ALL of your samples

- Sign the Safety Approval Sheet

Important: Please send to us any slides, images or publications of your results...
the more the merrier! Bon courage!