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Microfluidic laboratory


The microfluidics laboratory of SOLEIL is dedicated to the fabrication and analysis of microfluidic systems that are compatible with beamline applications. It benefits to a wide range of scientific domains, from solid-liquid interfaces to chemical reactions, single cell studies or protein crystallography. The availability of a microfluidic lab in SOLEIL is an opportunity that enables designing and preparing microfluidic systems adapted to the beamlines that would like to perform this type of in situ analyses.

The laboratory is equipped with state-of-the-art fabrication and analysis tools, in a dedicated clean room. The laboratory is accessible to all members of SOLEIL, as well as to external members that have a running project at SOLEIL or are planning to. The day-to-day organization of the lab and instruments maintenance is carried out by the members of the lab, but each user is responsible for realizing its own devices according to its project. Acces to the lab is granted after defining the proect with the lab manager and training on each instrument.

More details about the laboratory, its instruments and current projects can be found throughout this webpage. If you need more information than what you can find here, please do not hesitate to contact us.

Where to find the lab?


In-house users

SMIS : F. Borondics, S. Lefrançois, C. Sandt, P. Dumas

PX1/2 : L. Chavas, S. Sirigu, I. Chaussavoine, P. Montaville, G. Fox

SWING : Y. Liatimi, T. Bizien, J. Perez

DISCO : M. Refregiers, F. Jamme, V. Rouam

LaSU : S. Kubsky

BioLABS : G. David

External users

CEA - LIONS : P. Guenoun, C. Chevallard, F. Malloggi

Ecole Polytechnique - laboratoire de Biochimie: Yves Mechulam, Emmanuelle Schmidt, Pierre-Damien Coureux,Thomas Simonson, Alexey Aleksandrov


Beamline Scientist
Beamline Engineer Assistant
LIAO Yuan-Yuan
MATEO Tiphaine
Priam Jordan
Master student, Université Paris-Sud, Orsay.
Maurice Baptiste
Undergraduate student, Physical Measurements Technology Institute in Reims.


• Dr. Benedikt Lassalle

Benedikt was trained as a chemist at the Universités de Strasbourg and Université d'Aix-Marseille, France. He obtained his PhD in inorganic chemistry in 2008 at the Insitut de Chimie Moléculaire et des matériaux d'Orsay, under the guidance of Dr. Elodie Anxolabéhère-Mallart and Pr. Ally Aukauloo. His thesis was focused on the synthesis and characterization of biomimetic highly-valent manganese complexes, as intermediate models for the water oxidation reaction in Photosystem II (PS II). He then spent a year as a post-doc at the Commissariat à l'Energie Atomique in Saclay, where he worked on the electrochemistry of PS II in the team of Dr. A. Bill Rutherford. In 2009, He moved on to the Lawrence Berkeley National Laboratory in the team of Vittal Yachandra and Junko Yano, where he spent three years as a post-doc. He worked both on the study of PS II and related model complexes using X-ray spectroscopy at synchrotrons and free electron lasers and on the in situ/operando study of electrocatalysts for water splitting. In 2014, he became a beamline scientist on the LUCIA beamline at SOLEIL, where he is pursuing his work on the operando study of electrocatalysts in collaboration with several teams in France and throughout the world. Since 2015, he has been appointed at the head of the microfluidics Laboratory of SOLEIL, where he is combining microfluidic techniques with spectroscopy and  energy-related electrocatalysis.


• Dr. Yuan-yuan Liao

Yuan-Yuan Liao received a BsC in chemistry from the China University of Geosciences in Wuhan, in 2008. She then moved to France where she recieved a Master from the Université Paris-Sud in the SERP-Chem Erasmus Mundus program. She receieved her PhD in 2013 from the Ecole Normale Supérieure in Cachan, France, where she worked on the precipitation of organic nanoparticles under microfluidic conditions at the Laboratoire de Photophysique et de Photochimie. She went on for a two years post-doc at the Ecole Nationale Supérieure in Lyon, where she worked on the preparation of organic chromophores and their study with two-photon absorption spectroscopy. She then moved to the Commissariat à l'Energie Atomique in Saclay, where she studied the confinment of water molecules in imogolite nanotubes using IR spectroscopy.

Early 2018, she joined the microfluidic lab in the framework of the ANR project TREMTI. This project, in collaboration with the team of Dr. Yves Mechulam at the Ecole Polytechnique, aims at the development of microfluidic mixers for the preparation of cryo-EM time-resolved samples.


• Mr. Stéphane Lefrançois

After spending more than ten years at the national metrology office (BNM), Stéphane joined the SMIS beamline as an assistant engineer. For more than fifteen years, he has been developping Infra-Red optical systems for the SMIS beamline at SOLEIL as well as for other synchrotrons (ALBA and SESAME). He also developped microfluidic systems for biological IR imaging under physiological conditions for the MSIS beamline and for INRA laboratories. In 2017, Stéphane was promoted as an engineer and joined the microfluidic lab for part of his time, while continuing his work at the SMIS beamline.


• Ms. Tiphaine Mateo

Tiphaine is an engineer-apprentice, spending half her time in the Microfluidic lab and at the Polytech Paris-Sud engineering school. She is involved in microfabrication method development (lithography, metallic film deposition) and in specific projects with in house users. She is currently working on the development of microfluidic systems for the sequential electroreduction of CO2.


There are no open positions available at this time, but you are always welcome to enquire for internships.

Access to the lab

Access to the lab is reserved to SOLEIL members or users with a proposal accepted through SOLEIL's review panels (SunSet). External users interested in using the microfluidic lab should either contact the lab's responsible directly or a beamline scientist that is familiar with the lab. A short written notice describing the goals of the project and the instruments and ressources of the lab required might be asked for.

The fabrication of microfluidic devices, including their design and the choice of materials, is the responsibility of users, but the microfluidic team will be happy to orient and help to the best of its capabilities. Common and microfabrication-specific chemicals and consumables are available in the lab, but specific needs should be discussed and planned in advance.

The everyday maintenance tasks of the lab are performed by the lab's assistant, but each user is responsible for taking care of its own experiments and maintaning the lab in a decent situation.



Laserwriter (KLOE-Dilase 250): A high resolution (4µm) direct laser (375 nm) lithography system for versatile prototyping and maskless fabrication.

• Plasma cleaner (Henniker HPT-100): O2, Ar, N2 and air plasma cleaner.

• Insulating device (KLOE UV-KUB 2): UV light insulating device to be used with a mask.

• Spincoater (Laurell WS-650-23NPP): rotation speed, time and accelaration is programmable.

• Electronic cutting printer (Silhouette Cameo): This device allows cutting microfluidic spacers from thin sheets of plastic.

• 3D Printer (Objet30 Pro, Stratasys): Precision Desktop 3D Printing with PolyJet technology. Verowhite and Veroclear materials are available, with single layer thicknesses of 32 and 16 µm, respectively.

• Hotplates, chemical bench, sonicator, distilled water.

• Fume hood and laminar flow cabinet.



• Profilometer (gbs smart WLI): fast, non-contact 3D imaging system with micrometer resolution.

Macroscope (Leica DMS 1000).

Custom-made microscope 10x & 50x (equipped with a camera)

• pressure and flow controller (ELVESYS OB1 MK3): 3 independent channels (1 x 0-200 mbars, 2 x 0-2 bars) and flow sensors.

• Syringe and peristaltic pumps.

In House projects

• Trap-chips:

This project, carried out in common with the Proxima-1 team, aims at preparing micro-traps that can immobilize micro-crystals and position them precisely in an X-ray beam to collect diffraction data. This allows measuring data under physiological conditions and at room temperature. Sorting crystals or even cells for further in line anlysis is also a part f this project. Igor Chaussavoine, PhD candidate on PX1, Pierre Montaville, post-doc on PX1 and Leonard Chavas, beamline manager on PX1 are all involved in this project. A few pictures below show the chips made and the support used to position them on the PX-1 goniometer.



The SWING beamline is carrying out a project to develop in-line microfluidic mixers, which allow following chemical or biochemical reactions with WAXS. The dynamics of nanoparticles formation or protein folding reactions can all be tracked using this method. This project involves Thomas Bizien, beamline scientist on SWING and Youssef Liatimi, engineer assistant on SWING.


Sequential CO2 reduction:

This project aims at developping micro-reactors that can host electro-or photo-chemical reaction, which are arranged in series to form a sequential chemical synthesis system. We are particularly interested in the sequential reduction of carbon dioxide (CO2) into light hydrocarbons or basic chemical building blocks.

External collaborations


We have an active collaboration with Drs. Florent Malloggi, Corinne Chevallard and Patrick Guenoun to develop microfluidic cells adapted to IR and X-ray spectroscopies.


• Ecole Polytechnique, Biochemistry Laboratory:

We are collaborating with the team of Drs. Yves Méchulam and Thomas Simonson for the development of microfluidic mixers that can trap conformational intermediates in the protein folding reactions.

 01/03/2018: Yuan-Yuan Liao just joined the lab as a post-doc, welcome! During her PhD, Yuan-yuan used microfluidics to study the precipitation of organic nanoparticles using FLIM spectroscopy. In the framework of the ANR project TREMTI, and in tight collaboration with the team of Dr. Yves Mechulam at the Ecole Polytechnique, Yuan-yuan will work on the fabrication of microfluidic mixers for the preparation of cryo-EM time resolved samples.


 18/01/2018: The ivMX (in vivo Macromolecular Crystallography) satellite workshop of the SOLEIL User's Meeting was held in SOLEIL on January 16-17. Several members of the microfluidic lab attended and Igor Chaussavone gave a talk on his PhD work. Microfluidic tools are becoming instrumental in this nascent field: numerous developments in microfluidics are expected for diagnostic, selection, sorting and positioning of cells containing microcristals!


01/11/2017: Stéphane Lefrançois  is joining the team as an engineer, welcome to you! Stéphane has been and assistant engineer on the SMIS beamline for several years, where he developped IR optical systems as well as IR-compatible microfluidic devices. He has also design and setup several IR beamlines at other synchrotron light sources (ALBA, SESAME). He has just been promoted as an engineer and will share his time between the SMIS beamline and the microfluidics lab.


• 13/07/2017: The Microfluidic lab, together with the PX1 team and the Biochemistry Laboratory at Ecole Polytechnique has been awarded an ANR Grant to develop microfluidic mixers fro the study of transient biochemical processes. Watch for the post-doc job opening!


• 10/06/2017: Tiphaine defended her first year project and recieved 18.5/20. Congratulations!


• 03/04/2017: Baptiste Maurice joins the lab as an undergraduate intern. He will develop a flow cell adapted for Hard X-ray transmission and fluorescence measurements.


• 19/12/2016: Upgrade du dilase250, amélioration du software et des performances du laserwriter. Nous avons fait des tests pour le comparer à son ancienne version : il y a beaucoup moins de problème pendant l'impression, et le logiciel qui pilote la machine est plus intuitif.



•05/10/2016: Tests en résolution sur l'imprimante 3D.

Petit canal (500µm) dans fine membrane : 


• 05/09/2016: L'imprimante 3D vient d'être installée! Nous pouvons maintenant produire des systèmes sub-millifluidiques en un bloc, et la machine travaille pour nous!


• 01/09/2016: Bienvenue à Tiphaine MATEO qui nous rejoint pour les 3 prochaines années! Elle est apprentie-ingéneure à l'école Polytech Paris-Sud et aidera les utilisateurs à mettre en place leurs projets au sein du laboratoire. A part la microfluidique, Tiphaine aime l'escalade, le skate-board et les minéraux.


• 12/05/2016: Ecoulement laminaire de deux solutions colorées au sein d'un canal de 20µm de large d'une puce PDMS/verre.





• 15/01/2016: Première réalisation de lithographie avec le laserwriter!



Lab members in June 2017.
From left to right: Baptiste Maurice, Tiphaine Mateo, Benedikt Lassalle.


Baptiste is cleaning wafer, Tiphaine is testing a chip and Benedikt checks the patterns of a mold using the profilometer.


Baptiste checks the channels of a freshly made 3D-printed cell.


Tiphaine checks a resin pattern on a wafer.


Benedikt uses the optical profilometer to measure the dimensions of a mold after the lithography process.


Rain may be falling, Tiphaine keeps focused on her channels.


Photos credits Vincent Montcorgé.