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Microfluidic laboratory

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The microfluidics laboratory of SOLEIL (MF Lab) is dedicated to the fabrication and analysis of microfluidic systems that are compatible with beamlines applications. It benefits to a wide range of scientific domains, from solid-liquid interfaces to chemical reactions, single cell studies or protein crystallography. The availability of a microfluidic lab in SOLEIL is an opportunity that enables designing and preparing microfluidic systems adapted to the beamlines that would like to perform this type of in situ analyses.

The laboratory is equipped with state-of-the-art fabrication and analysis tools, in a dedicated clean room. The laboratory is accessible to all members of SOLEIL, as well as to external members that have a running project at SOLEIL or are planning to. The day-to-day organization of the lab and instruments maintenance is carried out by the members of the lab, but each user is responsible for realizing its own devices according to its project. Acces to the lab is granted after training on each instrument.

More details about the laboratotory, tis instruments and the project going on can be found throughout this webpage.

Team

LASSALLE
LASSALLE Benedikt
Beamline Scientist
MATEO
MATEO Tiphaine
MAURICE
Priam Jordan
Master student, Université Paris-Sud, Orsay.
Maurice Baptiste
Undergraduate intern

Where to find the lab?

Localisation:

In house users

SMIS : F. Borondics, S. Lefrançois, C. Sandt, P. Dumas

PX1/2 : L. Chavas, S. Sirigu, I. Chaussavoine, P. Montaville, G. Fox

SWING : Y. Liatimi, T. Bizien, J. Perez

DISCO : M. Refregiers, V. Rouam

LaSU : S. Kubsky

BioLABS : G. David

CEA - LIONS : P. Guenoun, C. Chevallard, F. Malloggi

Access to the lab

External users interested in the using the microfluidic lab should either contact the lab's responsible directly or a beamline scientist that is familiar with the lab. A short written notice describing the goals of the project and the instruments and ressources of the lab required might be asked for.

The fabrication of microfluidic devices, including their design and the choice of materials, is the responsibility of users, but the microfluidic team will be happy to orient and help to the best of its capabilities. Common and microfabrication-specific chemicals and consumables are available in the lab, but specific needs should be discussed in advance.

The everyday maintenance tasks of the lab are performed by the lab's assistant, but each user is responsible for taking care of its own experiments.

Instruments

Fabrication

• Laserwriter (KLOE-Dilase 250): A high resolution direct laser lithography system for fast prototyping and maskless fabrication.

• Plasma cleaner (Diener Femto): O2 plasma cleaner in collaboration with SOLEIL's Surface Lab.

• Insulating device (KLOE UV-KUB 2)

• Spincoater (Laurell WS-650-23NPP): rotation speed, time and accelaration is programmable.

 

• 3D Printer (Objet30 Pro, Stratasys): Precision Desktop 3D Printing with PolyJet technology.

• Electronic cutting printer (Silhouette Cameo): Design and cut your own custom mask.

• Hotplates, chemical bench, sonicator, distilled water,...

• Fume hood

 

Analysis

• Profilometer (gbs smart WLI): fast, non-contact 3D imaging system with micrometer resolution.

• Custom-made microscope 10x & 50x (equipped with a camera)

• Pressure & flow controller (ELVESYS OB1 MK3): 2 channels microfluidic flow controller with flow sensor.

• Precision syringe and peristaltic pumps.

In House projects

Trap-chips:

This common project with the Proxima 1 (PX1) team deals with the development of micro-traps for the immobilization of microcristals and their study by ambient temperature and physiological conditions X-ray diffraction. This project is run by Igor Chaussavoine (PhD student), Pierre Montaville (post-doc) and Leonard Chavas (beamline responsible) on PX1 and Tiphaine Mateo (engineer) from the Microfluidic lab.

 

TR-WAXS:

This project is run by the team of the SWING beamline and deals with the preapration and use of microlfuidic mixers that allow studying protein folding of nanoparticle synthesis by time-resolved Wide Angle X-ray Scatterring. This project is run by Thomas Bizien (beamline scientist) and Youssef Liatimi (beamline technician).

 

Sequential CO2 reduction:

This project is specific to the microfluidic lab and tries to develop microfluidic reactors for the sequential reduction of CO2 into valuable chemical or fuels. 3D-printed electrochemical and chemical microreactors set in line will be used to perform sequential, liquid phase catalytic reactions.

External collaborations

• CEA LIONS:

We have an active collaboration with Drs. Florent Malloggi, Corinne Chevallard and Patrick Guenoun to develop microfluidic cells adapted to IR and X-ray spectroscopies.

 

• Ecole Polytechnique, Biochemistry Laboratory:

We are collaborating with the team of Drs. Yves Méchulam and Thomas Simonson for the development of microfluidic mixers that can trap conformational intermediates in the RNA translation process.

• 13/07/2017: The Microfluidic lab, together with the PX1 team and the Biochemistry Laboratory at Ecole Polytechnique has been awarded an ANR Grant to develop microfluidic mixers fro the study of transient biochemical processes. Watch for the post-doc job opening!

 

• 10/06/2017: Tiphaine defended her first year project and recieved 18.5/20. Congratulations!

 

• 03/04/2017: Baptiste Maurice joins the lab as an undergraduate intern. He will develop a flow cell adapted for Hard X-ray transmission and fluorescence measurements.

 

• 19/12/2016: Upgrade du dilase250, amélioration du software et des performances du laserwriter. Nous avons fait des tests pour le comparer à son ancienne version : il y a beaucoup moins de problème pendant l'impression, et le logiciel qui pilote la machine est plus intuitif.

 

 

•05/10/2016: Tests en résolution sur l'imprimante 3D.

Petit canal (500µm) dans fine membrane : 

 

• 05/09/2016: L'imprimante 3D vient d'être installée! Nous pouvons maintenant produire des systèmes sub-millifluidiques en un bloc, et la machine travaille pour nous!

 

• 01/09/2016: Bienvenue à Tiphaine MATEO qui nous rejoint pour les 3 prochaines années! Elle est apprentie-ingéneure à l'école Polytech Paris-Sud et aidera les utilisateurs à mettre en place leurs projets au sein du laboratoire. A part la microfluidique, Tiphaine aime l'escalade, le skate-board et les minéraux.

 

• 12/05/2016: Ecoulement laminaire de deux solutions colorées au sein d'un canal de 20µm de large d'une puce PDMS/verre.

 

 

 

 

• 15/01/2016: Première réalisation de lithographie avec le laserwriter!