➊
Ionization efficiency of peptides as a function of their hydropathicity (as indicated by their GRAVY index)
using three different ionization techniques: Electrospray (ESI), thermospray (TSP) and atmospheric pressure
photoionization (APPI).
➋
(
A
) Topology of the protein BmrA exhibiting six transmembrane domains and a nucleotide binding domain
(NBD). (
B
) The purified protein was loaded on a gel and stained using Coomassie blue. (
C
) Mass spectrometry
analysis after in-solution digestion of the protein BmrA under ESI or APPI conditions. The precursor ion m/z
1198.75 corresponding to the first transmembrane domain is labelled with a black arrow.
➌
(
A
) In-source fragmentation of a juxtamembrane peptide under APPI.
(
B
) The abundances of each a-, b-, c- and y- sequence ions were plotted as a function of the peptide sequence.
A
B
B
C
A
DISCO beamline
ASSOCIATED PUBLICATION
A. Bagag, J.-M. Jault, N. Sidahmed-Adrar,
M. Réfrégiers, Alexandre Giuliani*
and F. Le Naour
PLoS ONE 8 (2013) e79033
REFERENCES
[1] T.J. Stevens & I.T. Arkin, Proteins
39 (2000), 417
[2] S. Tan S et al. Proteomics 2008 8: 3924
[3] A. Giuliani et al. Nucl. Instrum.
Methods Phys. Res. B 279 (2012), 114
[4] A. Giuliani et al. J Synchrotron Radiat.
16 (2009), 835
*Synchrotron SOLEIL, l’Orme des Merisiers,
St Aubin BP48, 91192 Gif sur Yvette Cedex,
France & INRA, UAR 1008 CEPIA, Nantes,
France
CORRESPONDING AUTHOR
57
SYNCHROTRON
HIGHLIGHTS
2013
