➊
Diagram of the MS/MS technique based on activation by VUV synchrotron radiation.
➋
Nomenclature for MS/MS peptide
fragmentation (
a
). Patterns of IB5
7+
protein fragmentation after activation
at 6.4 and 16 eV (
b
), by CID and ECD (
c
).
➌
Localization of the B2 3'OG binding
site on IB5. The objects IB5
7+
and IB5•B2 3’OG
7+
were selected
and irradiated with 16 eV photons (
a
).
Comparison of the MS/MS 16 eV VUV
spectra identified specific
fragments in the IB5•B2 3’OG
7+
fragmentation spectrum in which
the m/z ratio had a mass difference
corresponding to that of B2 3’OG7+
compared with fragments from the
fragmentation spectrum of IB5
7+
(
b
).
CID activation of identified fragments
confirmed the presence of the ligand (
c
).
The map of B2 3’OG-carrying fragments
identified the 'KPQGPPPPPQGG'
sequence as a B2 3’OG binding
site on IB5.
DESIRS & DISCO beamlines
ASSOCIATED PUBLICATION
Photodissociation and dissociative
photoionization mass spectrometry of proteins
and noncovalent protein–ligand complexes
F. Canon*, A. R. Milosavljevi
ċ
, G. van der Rest,
M. Réfrégiers, L. Nahon, P. Sarni-Manchado,
V. Cheynier, and A. Giuliani
Angewandte Chemie International Edition
52(32) (2013) 8377
REFERENCES
[1] A. K. Dunker et al. Curr. Opin. Struct. Biol.
18 (2008), 756
[2] F. Canon et al. Am. Chem. Soc.
133 (2011), 7847
[3] A. R. Milosavljevic et al. J. Sync. Rad.
19 (2012), 174
[4] F. Canon et al. A. Angew. Chem. Int. Ed.
52 (2013), 8377
* INRA, UMR1324 Centre des Sciences
du Goût et de l’Alimentation, 21000 Dijon,
France
CORRESPONDING AUTHOR
61
SYNCHROTRON
HIGHLIGHTS
2013
