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SWING, Small and Wide angle X-ray scattering

For studying complex structure samples: macomolecules, nanomaterials, soft matter, tissues...

 

Website under construction... please be patient

By providing information on the structure of matter at scales varying between nanometer and micrometer, the beamline SWING will help answering the numerous questions related to soft condensed matter, conformation of macro-molecules in solution and composite materials in material sciences.

Our experimental set up allows small-angle X-ray scattering (SAXS) and wide-angle X-ray scattering measurements (WAXS) to be performed simultaneously in the 5-17 keV energy range, as well as grazing incidence small angle scattering (GISAXS). Anomalous scattering experiments will be facilitated. Emphasis will be put on the variety of samples that can be studied, solutions, gels, amorphous solids, crystallised solids and the corresponding diversity of sample environments.

Team

BARANOWSKI
BARANOWSKI Maciej
Post Doctoral Student
BIZIEN
BIZIEN Thomas
Beamline Scientist
DOURI
LIATIMI
LIATIMI Youssef
Beamline Engineer Assistant
PEREZ
PEREZ Javier
Beamline Manager
THUREAU
THUREAU Aurelien
Beamline Scientist
Meneau Florian
Brazilian Synchrotron Light Laboratory (LNLS), Campinas
Roblin Pierre
Laboratory of Chemical Engineering , Toulouse
Galindo-Gonzales Cecilia
Koutsioupas Alexandros
Evrard Guillaume

Technical data

Energy range

Between ~5 and 17 keV

Energy Resolution

~2 eV

Source

In-vacuum U20 undulator.
Source Size (sigma, μm): 388 (H) x 8.1 (V)
Source Divergence (sigma, μrad): 14.5 (H) x 4.6 (V)

Optics

Diaphragm at 11.7 m (1x0.5 mm2
Fixed exit DCM Si111at 20 m
Fixed incidence focusing KB at 22.5 m 
Sample position : 30 - 32 m
Detector / Sample Distance : 0.5 – 8 m

Sample Environments
Beam size at sample

Between 20x20µm2 (zone plate) to 450x20 μm2 FWHM in the experimental hutch

Flux on sample

1.1013ph/s @7keV, 1.1012 ph/s @16keV (with 500 mA ring)

Detectors

SAXS:

  • Eiger 4M (Dectris)
  • PCCD170170 (AVIEX), Gain > 3ADU/ph, Noise≈2ADU 

WAXS: 

  • Hybrid Pixel detector (Detector Group pool)
Detection chamber

Under primary vacuum, SAXS detector positions :
- 0.20 / + 0. 20 m (horiz), -0.20 / +0.20 (vert), 0.5 m / +6 m (along X-rays).

qrange

 

Dedicated sample environments have been developed to provide scientific communities with optimized experimental conditions.  For soft-condensed matter, numerous devices are available (home-made capillaries holders, Linkam THMS600, Anton Paar rheometer with custom-made Couette cells …). For structural biology, a unique SEC-SAXS HPLC system is routinely used for online purification connected to a quartz capillary placed under vacuum cell. Here are presented the most used devices for both soft-condensed matter and structural biology.

HPLC: SEC-SAXS

 

  • Sample volume: 10 to 100µL
  • Sample Concentration: 1 to 30mg/mL
  • Two independent circuits of purification (can connect two columns with 2 different buffers).
  • Combination of the SEC-SAXS system with MALS and RI is now available at user request.
  • Automated workflows for injections and data analysis
  • IspyB database

For more informations (SwingHPLCWeb.pdf)

 

AutoSampler for BioSaxs

  • Sample volume: 10 to 50µL
  • Sample Concentration: 0.2 to 10mg/mL
  • Sample every 2.5 min (pipetting, injection, measurement, cleaning and drying).
  • 96 vials capacity
  • No dead time between batch and SEC-SAXS experiments
  • Thermalized vials holder (5-10 to 60 °C)
  • Injection rates of : 10 to 100 µl,min-1
  • Injection possible up to 30 % glycerol in water / 250 g.l-1 BSA
  • Automated workflows for injections and data analysis
  • IspyB database.
For more informations (SwingRobotPIERREWeb.pdf)

 

High Pressure

in construction

Static Capillaries Holder

Static Capillaries Holder
    • Horizontal and Vertical positions
    • 20 capillaries of Ø 1,5 mm (Ø 1 mm possible)
    • 5 mm high scanning windows behind the capillaries
    • 5 mm gap between capillaries
    • Thermalization between 5-60 °C
    • Automated workflows for injections and data analysis

    For more informations (SwingStaticCapillariesHolderWeb.pdf)

     

    Circulation Capillaries Holder

    Circulation Capillaries Holder
    • 3 capillaries of Ø 1,5 mm
    • Capillaries are vertically spaced of 15 mm
    • 20 mm horizontal scanning length
    • Thermalization between 5-60 °C

    Circulation through the capillaries can be done using various setups :

    • Peristaltic pump
    • Swing injection robot
    • Syringe driver
    • Manual injection
    For more informations (SwingCirculationCapillariesHolderWeb.pdf)

     

    Gel Holder

    in construction

    Mixing and pipetting cell

    Mixing and pipetting cell
    • 5-10 ml mixing cell
    • sample pipetting through a Ø 1,5 mm quartz capillary
    • Possible Injection into the mixing cell through an injecting hole synchronized with detection
    • Thermalization possible between 5-10 °C to 60 °C
    • Automated workflows for injections and data analysis

     

    The sample is drawn in the capillary only and at the required height. After X-ray exposition, it’s reinjected into the mixing cell.

    For more informations (SwingMixingPipettingCellWeb.pdf)

     

    Linkam

    Linkam
    • Linkam THMS600
    • Temperature range: -196 to 600 °C
    • Up to 150°C/min heating and 100°C/min Cooling
    • Capillaries have to be Ø 1,5 mm maximum and cut in order to reach 20 mm length otherwise it won’t fit in the linkam cell
    • Dedicated linkam software
    For more informations (SwingLinkamWeb.pdf)

     

    Traction Cell

    Traction Cell
    • Max. Working load: 111 N
    • Max. working length : 6 mm
    • Resolution 0.05 N
    • Step : 0,5 µm
    For more informations (SwingTractionCellWeb.pdf)

     

    Rheometer

    Rheometer

    Rheometer Anton Paar MCR 501

    • X-ray transparent Couette cells
    • Couette cells are of 10 mm radius, a height of 17 mm and gaps values (Re – Ri) of 0.5 or 0.2 mm
    • Sample Volume is of about 1 ml
    For more informations (SwingRheometerWeb.pdf)

     

    Stop-Flow (+MALS)

    in construction

    Micro-fluidic

    in construction

    BioSaxs

    Before Coming

    What you have to bring

                    We use Agilent standards vials and inserts for the HPLC device and the Auto Sampler. We lend you the vials and the caps but you have to buy you own inserts (Agilent reference: 5181-1270). In any case, we can give some used inserts that you can wash and dry.

    What we can lend you

                    The biology laboratory (link) provides support to users for the preparation of their experiments. For any question, please ask the laboratory staff.

                    We recommend you to bring your own analytical columns for SEC-SAXS. We have also a wide selection of column for users. All these columns have been calibrated:

    BioSec3-100

    Column Volume:5mL

    Mass Range: 0.1 to 100kDa

    pH Range: 2-8.5

    biosec3-100 elution profile

    AdvBioSec 2.7-300

    Column Volume:5mL

    Mass Range: 5 to 1250kDa

    pH Range: 2-8.5

    biosec Advance 2.7-300 elution profile

    BioSec3-300

    Column Volume:5mL

    Mass Range: 5 to 1250kDa

    pH Range: 2-8.5

    biosec 3-300 elution profile

    BioSec 5-1000

    Column Volume:5mL

    Mass Range: 50 to 7500kDa

    pH Range: 2-8.5

    biosec 5-1000 elution profile

    BioSec 5-2000

    Column Volume:5mL

    Mass Range: >10000kDa

    pH Range: 2-8.5

    biosec 5-2000 elution profile

    During your session

    The session includes beamline set up installation. It’s recommended to discuss with your local contact before coming to the beamline to save time during installation.

    Running experiments

    Our GUI (passerelle) allows automatic data collections of multiples simple in a row. For direct data monitoring and security check, an others GUI (Cook) is installed.

    The local contact will show you how to use these GUI.

    Data Processing and analysis: Foxtrot & AutoBioSAXS

    All the images produced on the Swing beamline can be process by homemade software (Foxtrot) in collaboration with Xenocs. You can ask for it by email to Xenocs by mail.

    You can find here (new link) a tutorial for processing SEC-SAXS experiments.

    For SEC-SAXS and BioSaxs AutoSampler, an automatic workflow for data analysis is available. This workflow takes raw images as input files and sends all results curves into IspyB database. Furthermore all intermediates files are available for users and could be reloading in our Foxtrot application.

     Living area

    We have a living area where you can use coffee machine, Sofa, fridge for food and beverages, micro wave and table. Wifi is available using your sunset login and password or using Eduroam network.

    This area is sample free and please clean it after your session.

    After your session

    Please don’t forget to fill the end of report in the sunset webpage.

    All your datas are available using the Soleil data Retrieval via the sunset web page.

    ChemSaxs

    During your session

    The session includes beamline set up installation. It’s recommended to discuss with your local contact before coming to the beamline to save time during installation.

    Running experiments

    Our GUI (passerelle) allows an “on demand” complex data collections strategies. For most of the sample environment, a workflow is already written. For direct data monitoring and security check, an others GUI (Cook) is installed. The local contact will show you how to use these GUI.

    Data Processing and analysis: Foxtrot & AutoBioSAXS

    All the images produced on the Swing beamline can be process by homemade software in collaboration with Xenocs. You can ask for it by email to Xenocs (link).

     Living area

    We have a living area where you have coffee machine, Sofa, fridge for food and beverages, micro wave and table. Wifi is available using your sunset login and password or using Eduroam network.

    This area is sample free and please cleans it after your session.

    After your session

    Please don’t forget to fill the end of report in the sunset webpage.

    All your datas are available using the Soleil data Retrieval via the sunset web page.

     

    Downloads

    BioSAXS Course - Javier Pérez :  

    Foxtrot

    Foxtrot Software (Data reduction and treatment)

    Ask by mail

    Foxtrot tutorial :
    Foxtrot : F.A.Q.

    Denfert

    Denfert Software (Ab initio shape determination, including hydration layer)
    version 2.0 (June 2015)

    Denfert : F.A.Q.
    A zip archive containing executables for Mac, Linux and Windows together with a test example may be downloaded by clicking here.

    ... a program for the ab initio “dummy-atom” structural modeling of Biological Macromolecules including the contribution of their inherent hydration layer.

    Developed by Alexandros Koutsioubas and Javier Pérez

    If you use DENFERT, please cite :

    A. Koutsioubas & J. Pérez, Journal of Applied Crystallography (2013) 46, 1884 and A. Koutsioubas, S. Jaksch & J.Pérez, J. (2016). J. Appl. Cryst. 49  (doi:10.1107/S1600576716003393)

    Brief Description

    DENFERT is implementing a simulated annealing algorithm similar to DAMMIN program by D. Svergun (Biophys. J. 76, 2879-2886) for the restoration of low-resolution structural info of bio-molecules from SAXS and SANS data. The major advantage of DENFERT is that the hydration layer around bio-molecules is taken into account by introducing a second type of beads (hydration beads) in the model.

    In the top figure, we see an example of the shape restoration of Lysozyme from SAXS data using DENFERT. A cartoon representation of the crystallographic structure is also presented for comparison. The bottom figure depicts additionally the hydration layer around the reconstructed protein shape