The workshop on "In vivo Macromolecular Crystallography: Advances and Perspectives" (ivMX) aims to gather the experts and the wider scientific community around the subject of intracellular protein crystallisation. Well-encountered phenomenon for decades, in cellulo crystal appearance grew in interest since its application to the structure determination of macromolecules directly from their natural environment. Recent experimental approaches in favour of a better handling of these complex systems greatly participated in the possibilities in studying the samples with less mechanical stress and sample loss. In this context, new developments in x-ray sources and microfluidics have been the key to the proper opening of these approaches to the wilder scientific community. The crystal growth of macromolecular objects directly from within their natural environment allows to directly link function to structure; with great medical, pharmaceutical and thus societal ramifications.
During this workshop, we will introduce new approaches for in vivo crystal preparation on various types of samples, the methods dedicated for their handling, and all the state-of-the-art strategies for sample characterisation and data collection at various synchrotron-based instruments. Experimental results will be brought forward in lights of new generation x-ray sources, fully related to the scientific needs for structural biology at SOLEIL. New instrumentation and microfluidic developments will be discussed, together with data collection strategies linked to it.
The ivMX 2018 workshop is organised by the Synchrotron SOLEIL, where it will be held as a satellite of the Users' Meeting 2018.
Scientific Committee
- Léonard CHAVAS ;
- Pierre MONTAVILLE.
Local Organizing Committee
- Clémentine ESNARD ;
- Frédérique FRAISSARD ;
- Jean-Marc LUCACCHIONI ;
- France POCHARD.
Invited Talks
- Jacques-Philippe COLLETIER, IBS, France
- Fasseli COULIBALY, Monash University, Australia
- Subramanian RAMASWAMY, INSTEM, India
- Lars REDECKE, Lübeck University, Germany
- Savvas SAVVIDES, Ghent University, Belgium
- Hidekazu TSUTSUI, JAIST, Japan
Programme
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Tuesday 16th January 2018 |
|
|---|---|
| 11:30 - 14:00 | Registration |
| 13:00 - 14:00 | Welcome coffee |
| 14:00 - 14:40 |
Setting up an In-vivo crystallography platform at SOLEIL |
| 14:40 - 15:20 | Invited Talk Mammalian crystalline proteins in vivo see the light Savvas Savvides - Ghent University, Belgium |
| 15:20 - 15:40 |
Short talk |
| 15:40 - 16:00 | Short talk Pulse-chase experiments to study in vivo crystal formation in a Coccolithophore Benedikt Lassalle - Synchrotron SOLEIL, France |
| 16:00 - 16:40 | Coffee break and group photo |
| 16:40 - 17:20 | Invited Talk Structure of a heterogeneous, glycosylated, lipid-bound, in vivo grown protein crystal at atomic resolution Subramanian Ramaswamy - INSTEM, India |
| 17:20 - 18:00 | Invited Talk In vivo-grown protein microcrystals: the arms and armour of insect viruses Fasseli Coulibaly - Monash University, Australia |
| 19:00 - 21:00 | Aperitif and dinner at SOLEIL canteen |
| Wednesday 17th January 2018 | |
| 08:30 - 09:00 | Coffee Break |
| 09:00 - 09:40 | Invited Talk Artificial photo-convertible fluorescence proteins: engineering, mechanism, and in vivo crystallization Hidekazu Tsutsui - JAIST, Japan |
| 09:40 - 10:20 | Invited Talk A potent binary mosquito larvicide revealed by de novo phasing with an X-ray free-electron laser Jacques-Philippe Colletier - IBS, France |
| 10:20 - 11:00 | Coffee break |
| 11:00 - 11:40 | Invited Talk Protein Crystallography in Living Insect Cells Lars Redecke - Lüdbeck University, Germany |
| 11:40 - 12:10 | Invited Talk Second-order Nonlinear Imaging: Application in Detection and Characterisation of Protein Crystals Frederic Jamme - Synchrotron SOLEIL, France |
| 12:10 - 12:40 | Invited Talk Microfluidic Positioning of biological macromolecular crystals for serial X-ray Diffraction on the Proxima-1 Beamline Igor Chaussavoine - Synchrotron SOLEIL, France |
| 12:45 - 14:00 | Lunch at SOLEIL |
| 14:00 - 15:00 | Round Table |
| 15:00 - 16:30 | Visit of Synchrotron SOLEIL |
Registration online
Registration is closed.
The registration fee is 80,00€ (V.A.T included).
It includes: attendance at the users meeting, buffets, lunches and coffee break.
| TYPE OF PARTICIPANT | AMOUNT (V.A.T included) |
|---|---|
| Researcher, institutionnel, post-doc | 80,00€ |
| Student, Invited speaker, Organisation Committee member, SOLEIL staff | Free |
Terms of payment
PLEASE NOTE : Order forms are not accepted
Payment by cheque :
Would you please write it out to “Synchrotron SOLEIL” and write at the verso the following reference:
Satellite workshop ivMX 2018 / 69170605.
Thank you for sending your cheque at the address, here below, together with the registration confirmation sheet or the e-mail of confirmation:
Synchrotron Soleil
Bureau des Utilisateurs de SOLEIL
L'Orme des Merisiers
Saint Aubin - BP 48
91192 GIF SUR YVETTE Cedex
Payment by bank transfer :
Thank you for transmit your payment by bank transfer to the following account number:
BNP Paris Bas
International Bank Account Number (IBAN) : FR76 FR76 3000 4025 5200 0106 0374 407
Code d'identification bancaire (BIC) : BNPAFRPPIFO
Synchrotron SOLEIL
with mentionning your name and the users meeting reference: Satellite workshop ivMX 2018 / 69170605
Your registration will be definitive as soon as your payment will be received.
Payment by credit card :
A payment online is possible.
You just have to select this mode of payment when registrating and to follow instructions.
Or, if you are already registered, by clicking on the link.
Oral presentation
Deadline for abstract submission: November 27th, 2017
If you wish to contribute, you can submit your abstract:
- in the registration form
- or otherwise when submitting an abstract
You will be informed about the selection results from the Programme Committee.
Invited Talks
Deadline for abstract submission: January 8th, 2018
Invited speakers are requested to submit their abstract:
- in the registration form
- or otherwise when submitting an abstract
Posters
Deadline for abstract submission: January 8th,2018
A posters session will be held on January 17th 2018.
Posters panels will be located in the Hall of SOLEILCentral Building.
Panels’ size: 180cm x 130cm
If you wish to contribute, you can submit your abstract:
- in the registration form
- or otherwise when submitting an abstract
|
In both cases, you are requested to use ONLY the template below (Word or .Rtf file - Any abstract not in compliance with the template will be sent back to the author):
The size of the files is limited to 4 Mo. |
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