Accelerated structural genomics on proteins coded by the pathogen agent of CoViD-10 using in vivo macromolecular crystallography
We propose to clone all the proteins coded by the pathogen agent responsible for the CoViD-19, and to use these full lengths and domain-truncated forms in our pipeline for accelerated genomics. The pipeline relies on the growth of protein crystals in vivo and in cellulo, directly within mammalian cells. Through its elementary concept, it permits a speed up in protein crystallisation while keeping in a locked-down system. More information on the pipeline and its disponibilities will be communicated shortly.
Following expressions and crystal growth, the structures will be solved using serial crystallography approaches recently developed at synchrotron sources. Positive results will then serve as potential targets for the conception of new structure-based drugs. These progresses shall help understanding better the virus and its pathogenicity, necessary steps toward the conception of medical treatments of antiviral molecules against the larger family of coronaviruses, on the basis of the structural knowledge of SARS-CoV-2 at atomic resolutions.
