Second BioXAS Study Weekend
Genomics and BioXAS

June 29 - 30, 2003

Programme
JUNE 29, 2003
Starting 9:30
Introduction
9h:30 I. Ascone - Overall goals of BioXAS Study Weekends, why and how to go forward.
Section 1 - Present status and forthcoming structural genomics initiatives: traditional and high throughput expression of metalloproteins
9:45 – 10:15 Gary Sawers (John Innes Centre, Norwich, UK)
Overproduction of recombinant copper proteins in Escherichia coli.
10:15 - 10:45 John Hall (De Montfort University, Leicester, UK)
High throughput expression of metalloproteins from the TB genome.
10.45 - 11:15

Coffee break

11:15 - 11:45 Frank E. Jenney (South East Structural Genomics, Georgia, USA)
HTP - multifaceted expression and protein purification system.
11:45 - 12:15

Yuki Yokohama (RIKEN Structural Genomics Centre, Japan)
Cell free expression system for metalloproteins.
12:15 - 14:15 lunch (cafeteria Amphithéâtre P. Lehmann)

Section 2 - Theoretical developments and computer codes for BioXAS

14:15 - 14:45 John Rehr (University of Washington, USA)
Bayes-Turchin approach to the analysis of BioXAS.
14:45 - 15:15 Maurizio Benfatto (INFN, Frascati, Italy)
Advances in the quantitative structural analysis of XANES spectra: biological applications.
15:15 - 15:45

Keisuke Hatada (INFN Frascati Italy and Chiba University, Japan)
Theoretical calculation of self-consistent optical potential based on GW scheme.
15:45 - 16:15 Coffee break
16:15 - 16:45

Grant Bunker (Illinois Institute of Technology, USA)
Calculation of multiple scattering Debye Waller factors in metalloproteins
16:45 - 17:15 Peter Kruger (ESRF, France)
Theory of Ca-L2,3 edge - XAS using novel multichannel multiple scattering method.
Section 3 - Instrumentation for Genomics BioXAS
17:15 - 17:45 José Goulon (ESRF, France)
Selected instrumentation and detector developments at the ESRF beamline ID12.
17:45 - 18:15 Gareth Derbyshire (Rutherford Appleton Laboratory, UK)
Germanium multi-element fluorescence detectors. A systems approach
18:15 - 18:30

 Isabella Ascone (LURE/LCBB, Orsay, France)
Control of protein redox state during synchrotron X-ray irradiation.
18:30 - 18:45

 Keith Hodgson (SSRL, Stanford, USA)
First results with the new 100 fsec source at SSRL.
18:45 - 20:00 Poster session (Hall Amphithéâtre P. Lehmann)
20:00 Social dinner (cafeteria Amphithéâtre P. Lehmann)
22:00 end
   
JUNE 30, 2003

Starting 9:00
Section 4 Recent scientific achievements and high -throughput BioXAS
9:00 - 9:40

James Penner - Hahn (University of Michigan, USA)
On the prospects for high - throughput XAS studies using third - generation synchrotron sources.
9:40 – 10:20 Robert Scott (University of Georgia, USA)
Bottlenecks and roadblocks in high - throughput XAS for structural genomics.
10:20 – 10:40 coffee break
10:40 – 11:20

Britt Hedman (SSRL, Stanford, USA)
Structural genomics - crystallography and XAS - separate and combined.
11:20 -11:50 Holger Dau (Freie University, Berlin, Germany)
BioXAS to watch biological catalysis
11:50 – 12:10 Martin Feiters (University of Nijmegen, The Netherlands)
EXAFS Studies of the Iodine and Bromine metabolism in brown algae
12:10 -14:00 lunch lunch (Bures University restaurant, self-service section)
14 :00 -15 :00 Poster session (Hall Amphithéâtre P. Lehmann)
Section 4 Recent scientific achievements and high -throughput BioXAS (continued)
15:00 -15:40

Stefano Mangani (University of Siena, Italy)
Integration of XAS and NMR for quick structure determination in structural genomics projects.
15:40 – 16:10 Wolfram Meyer-Klaucke (EMBL, Hamburg, Germany)
ZiPD - a new enzyme with a well - known fold
16:10 -16:40 Coffee break
16:40 - 17:10

Uwe Heinz (University of the Saarland, Saarbruecken, Germany)
Recent EXAFS studies on metallo-beta-lactamases
17:10 – 18:00 Round table
Chair R. Fourme Participants : I. Ascone, M. Benfatto, S. Hasnain, K. Hodgson, Z. Wu
Coordinated actions for the development of BioXAS (SR facilities and teams) and to make XAS available to a wider biological community. Initiatives in Europe, USA and other countries
Ziyu Wu (BSRF, Beijing, China) : Sino-Euro programme for BioXAS
18:30 End of the meeting