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PROXIMA 1 beamline

Research Contents / Beamlines / PROXIMA1

PROXIMA 1 is one of two beamlines at SOLEIL for measurements in macromolecular crystallography (together with PROXIMA 2, recently opened to users). PROXIMA 1, operational since March 2008, delivers an intense, parallel and tunable x-ray beam for measurements at high resolution or from large unit cell dimension crystals. The beamline has recently been upgraded with a very large surface area detector (PILATUS 6M). The 3 circle "kappa" geometry goniostat is in routine use for advanced data collection strategies. A new CATS sample changing robot has been installed on the beamline and is operational for cryogenically cooled samples. A crystallisation plate screening option will be available soon. The beamline optics implemented are such that they could permit a range of focussing conditions which can then be adapted to the sample being studied (for example beam focus at sample or detector).

 
RNA splicing - the lariat conformation captured at PROXIMA-1!  
 

Group II introns are large catalytic RNAs and mobile genetic elements of bacterial origin. They are considered to be the ancestors of nuclear introns in eukaryotes and their splicing machinery, the spliceosome. In both splicing systems the intron is released in a ‘lariat’ conformation carrying a specific 2’-5’ phosphodiester bond. X-ray diffraction data collected at PROXIMA-1 beamline by scientists from the Institute for Integrative Biology of the Cell (I2BC) allowed to solve two crystal structures of a group II intron lariat primed for reverse splicing. These structures illuminate the raisons for the extreme conservation of the lariat conformation during the evolutionary path from group II to spliceosomal splicing. This work was published in Science, December 2, 2016. [Full story]


 
 
  More information | All the news
Description de la ligne Proxima 1

Thématiques scientifiques | Données techniques | L'équipe

Practical informations for users

Before the experiment | Troubleshooting | Users manual

Workshops

Workshop on Advanced Data Collection with Multi-Axis Goniometry

AtelierBioCrystallographie2016

 Beamline scientific articles

 Beamline PDB Entries

 News

- PROXIMA 1 - PROXIMA 2A : The crystallographic structure of the enzyme PolD suggests a new paradigm for classification of DNA polymerases

- Industrial anti-cancer research, assisted by diffraction data collected at SOLEIL, is reported in the journal Nature

- PROXIMA1 - PROXIMA2A: Regulation of gene expression - Take off your cap! 

- Structure of colloidal crystals

- The structure and the nutritive power of cockroach milk crystals

All the beamline news

 

 

 

 General information:

- Preliminary Project

- Beamline description 

Beamline poster

- PROXIMA2 Beamline

Download diagram of the energy ranges

 

 

How does the protein synthesis start in the cells?
The 3D structure of the e/aIF2 factor, a major actor of this stage, has been solved

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