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SRMS2 est l'acronyme de Synchrotron Radiation for Tandem Mass Spectrometry. Ce projet (N° BLAN08-1_348053) bénéficie du soutien de l'Agence Nationale pour la Recherche pour une durée de deux ans à compter de début 2009.
Nous mettrons à jour au fur et à mesure les progrès effectués sur ce projet.
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Les partenaire du projet sont d'une part l'INRA et d'autre part le Synchrotron SOLEIL.
Problème posé :
Modern ionisation techniques have established mass spectrometry as central method for analysis of a wide range of samples. In biological analysis, the low sample consumption of MS and its ability to determine the structure of bio-polymers is particularly appreciated. Very often, primary structures of biological molecule are determined using tandem mass spectrometry (MS2), a particular arrangement in which ions of interest are dissociated into their components. Classical methods fail for example to cleave large biomolecules. There is a clear need for new MS2 methods able to release large amount of internal energy into the ions on interests. A simple and straightforward mean to achieve this is absorption of energetic photons by the target, typically in the vacuum ultra violet (VUV).
Unfortunately, this is not a trivial task, because of difficulties in achieving high enough ion densities and photon fluxes.
However, we demonstrate in this project that irradiation with VUV photons and subsequent fragmentation of protein is possible at third generation synchrotron radiation facilities for ions stored in ion traps. The coupling of ion trap and VUV beamlines opens up the door to the study of electronic excited states of ions. We believe that important profits may be gained from the population of excited states of proteins. The electronic spectroscopy of poly-protonated proteins ions is very likely to find applications for their structural analysis. Moreover, the photochemistry of small ligand molecules forming non-covalent complexes with proteins might also be profitable to this field.
This project is concerned with coupling of a linear quadrupole ion trap on two VUV beamlines in the 5 to 20 eV at the Soleil synchrotron, to study the spectroscopy of electrosprayed ions. This is an almost virgin domain because this proposed combination of techniques is unprecedented. This groundbreaking approach could yield to new methods for structural determination in the gas phase. It could be extended later on to higher photon energy range.
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