 Endstations descriptions
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- SRCD: allows to record circular dichroism spectra in solution down to 165 nm even with very aborbing buffers and films down to 120 nm. Very fast acquisition of datas about folding and stability of biomacromolecules.
- POLYPHEME: two microscopes
• a microspectrofluorimeter (POLYPHEME) for recording fluorescence spectral images of molecules absorbing between 190 and 400 nm. allows femtoliter spectral recording.
• a full field, fully automatized inverted microscope, for fast imaging of live cells or any other specimen that do present fluorescence in UV. Often used in Z-Stacks configurations. - Numerous molecules present a specific fluorescence when excited in UV (190 to 400 nm), here are some examples that we did encounter: aromatic amino acids of course, but also NADH, DHE (that may be sustitued to cholesterol for studying membranes), ferulic acids, lignin, G quadruplexes, polyenes, metal oxydes, ...
- APEX: to ionize at atmospheric pressure the biomolecules between 5 and 20 eV. This ionization can be coupled with a mass spectrometer.
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Projects Submission Help
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- N'oubliez pas de contacter un des scientifiques de la ligne.
- Les domaines d'énergies dépendent des branches
- Les environnements échantillons sont définis avec les scientifiques de ligne.
- La justification du temps de faisceau demandé est cruciale.
- Comment citer la ligne et SOLEIL dans vos articles après les expériences ?
Nous vous recommandons de
• Citer la ligne dans les matériels et méthodes (DISCO beamline), avec la référence à Giuliani et al, Journal of Synchrotron Radiation 2010;
• Citer la branche sur laquelle vous avez travailler avec la référence en rapport;
• Remercier SOLEIL dans les acknowledgement, avec le ou les numéros de projets liés à vos expériences;
• Remercier le local contact si vous le souhaitez.
N'oubliez pas de mentionner vos publications liées à la ligne dans le sunset.
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Lexicon
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| DISCO | | Dichroism, Imaging and mass Spectrometry for Chemical and biOlogical systems (Inspired author : L. Nahon)
| | CD BIO | | Old name of DISCO
| | CD | | Circular Dichroism
| | SRCD |
| Synchrotron Radiation Circular Dichroism | | APEX | | Atmospheric Pressure Photoionisation EXperiment | | POLYPHEME | | Cyclop was already taken... this is DISCO Microscope nickname, son of Poseidon he does'nt know nobody
| | TELEMOS | | Another Cyclop with the gift of telling the future, son of Protée
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 Websites & publications of interest
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Users Publications
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Lien vers les publications des utilisateurs (signalées par un U).
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Related Topics
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Complementary beamlines at SOLEIL
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DISCO experiments is complementary to SMIS (Infrared microscopy) for cellular biology. SRCD and Mass spectrometry are complementary to SWING, PROXIMA I and PROXIMA II for structural biology and biochemistry.
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Other SRCD beamlines in the world
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Active
| B23, Diamond | UV1, ASTRID | U9B, NSLS | 3m NIM1, BESSY2 | BL15, HiSOR | | Oxon ,UK | Aarhus, Dk | Brookhaven, USA | Berlin, DE | Hiroshima, JP |
In construction
BESSY 2 new
| NSRL | CD12 revamped | 3B1B, BSRF | Berlin, DE
| Hefei, CHINA | Anka | Beijing, CHINA |
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Our users do have talent !
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