 Scientific Opportunities
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DISCO is a VUV to visible beamline of phase 2. First users were received in september 2009.
Three endstations are operated around a common scientific topic : Biomolecular investigations, with special emphasis on proteins, particularly membrane proteins:
- A circular dichroism endstation in the VUV range which opens new fields in the investigation of biological molecules, with, in particular, the possibility to follow rapid kinetics such as proteins folding and unfolding in real time,.
- An endstation for mass spectrometry of non-soluble molecules based on VUV photoionization with new opportunities for proteomics and photochemistry of hydrophobic molcules.
- An imaging endstation for biological (living cells) and material applications with new possibilities of excitation and detection, also allowing biomedical investigation of normal and tumoral tissues moreover, new paradigms in autofluorescence diagnosis appear and "abandonned" molecules that fluoresce only in the UV can be used as probes.
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| Material sciences | | Microscopic Analysis defaults inside optical materials ; prebiotics and fossils inclusions topology inside geological materials.
| | Biochemistry | | Proteins and DNA structures and dynamics ; sugar structures ; Mass spectrometry of proteins
| | Biology , Biomedics | | Imaging biochemical reactions inside individual cells with fluorescent proteins; Autofluorescence of tumoral cells and tissues; Study of drug distribution inside human tissues without additional labelling ; diagnosis of hydrophobic molecules
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Team
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Slavka Kascakova
Post-doc Imaging branch
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| Ha-Phuong Ta
Post-doc SRCD | |  |
| | | | | | | | Associés | | | Alain Buléon
DR INRA | |  | Alain Brunelle
Team Leader, ICSN | | | Sandrine Villette
CBM | |  |
Adresse postale
| Synchrotron SOLEIL
L'orme des Merisiers
Saint-Aubin - BP48
91192 GIF-sur-YVETTE CEDEX | |
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 Former team members
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Nom
| Ancien rôle à DISCO
| Actuellement
| | Simona Miron | Post doctorante SRCD | CR Inserm | | Aleksandar Milosavljevic | Post-doctorant ANR SRMS2 | Research assistant Professor
Institute of Physics of Belgrade,
| | Francis Canon | Post-doc APEX | CR INRA | | Youssouf Diouf | Master SRCD | | | Luba Buriankova | Doctorante Polyphème | Kosice Univ, Sk | Huang Yan
| Visiting scientist | Scientist, BSRF | | Sirinart Srichan | Doctorante avec SMIS | Research Assistant at SLRI
Thaï Synchrotron
| | Clara Foussat | Stagiaire SRCD | Elève ingénieur | | Lavanya Premvardhan | Post-doctorante
| webpage | | David Guery | Stagiaire APEX | Master |
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Technical data
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| Optiques | Flat and toroid mirrors with cold finger for M0. Beam divided in three for the endstation
| Source
| Dipole Radiation: 40 mrd H x 10 mrd V
| Branche
| SRCD | APEX | POLYPHEME
| Domaine d'énergie
| 120-400 nm | 60-220 nm | 180-1000 nm
| | | | | | Résolution en énergie
| 0.1 nm
| 0.1 nm | 0.5 nm
| | Flux @ first optical element | | | 10 e+14 Phot/s/0.1%bw @ 100 nm
| Environnement échantillon
| Cellules en CaF2
| Variable | Inverted microscope with spectral and temporal resolutions with controled temperature and CO2
| Taille de faisceau
sur l'échantillon
| 4x4 mm2
| 4x4 mm2 | 0.1x0.1 μm2 | Detecteurs
| PMTs | mass spectrometer | PMTs, time gated CCD | | Polarisation | Circulaire | |
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Optical design
DISCO beam is extracted from a dipole : D04.1 (1.71 T). A 40 per 10 mrad opening is taken.
Setup
The imaging endstation comprises two inverted microscope with pre and post monochromators and three detectors : one PMT, one phase gated CCD camera and one APD.
The mass spectrometry endstation comprises one commercial mass spectrometer with an injection port and an analysis station.
The CD endstation comprises one optical bench with a photoelastic modulator, a sample holder and a PMT.
Other facilities
A tiny lab dedicated and very close to DISCO includes a cell laboratory and a mini biochemistry lab.
The huge biochemistry lab for users is located closely undercover from the beamline.
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